Archives of Pediatric Infectious Diseases Archives of Pediatric Infectious Diseases Arch Pediatr Infect Dis http://www.pedinfect.portal.tools 2322-1828 2322-1836 10.5812/pedinfect en jalali 2019 9 17 gregorian 2019 9 17 5 2
en 10.5812/pedinfect.37680 Diabetic Foot Infections: Antibiotic Susceptibility Patterns and Determination of Antibiotic Cross-Resistance in Clinical Isolates of Enterococcus Species During 2012 - 2014 in Shiraz, Iran Diabetic Foot Infections: Antibiotic Susceptibility Patterns and Determination of Antibiotic Cross-Resistance in Clinical Isolates of <italic>Enterococcus</italic> Species During 2012 - 2014 in Shiraz, Iran research-article research-article Conclusions

Our data showed that resistance to vancomycin among Enterococcus spp. isolates is emerging. Knowledge of the causative microorganisms in DFIs and their antibiotic susceptibility profiles is essential for proper treatment and infection eradication.

Results

In the current study, a total of 86 diabetic patients were investigated. Enterococcus spp. were isolated from 34 (39.5%) patients consisting of 20 males (59%) and 14 females (41%). Twenty-five (73.5%) patients received antibiotic treatment on admission. Fifty (44.1%) cases had random blood sugar ranging between 130 - 300, and 19 (55.9%) had blood sugar of 300 - 450. Of the 34 patients, 15 (44.1%) had type 1 diabetes and 19 (55.9%) had type 2 diabetes. Enterococcus faecalis was the most common isolated Enterococcus spp. (50%). Linezolid was the most effective antibiotic against Enterococcus isolates, and ciprofloxacin was the least effective.

Background

Diabetic foot infections (DFIs) are an increasingly common public health problem and are associated with mortality and morbidity. The incidence of Enterococci in DFIs, a leading cause of hospital admission in Iran, has been increasing, possibly due to previous antibiotic use.

Objectives

The aims of this study were 1) isolation of bacteria from diabetic patients with foot ulceration, 2) characterization of the isolated bacteria, 3) confirmation of Enterococci and their genus, 4) determination of the susceptibility profile of the isolates, and 5) survey of the cross-resistance among Enterococcus spp.

Methods

A total of 86 diabetic patients with foot ulceration were investigated during 2012 - 2014 in Nemazee hospital (Shiraz, Iran). Swabs were collected from diabetic ulcers. For the isolation of bacteria, microbiological media were used. Colonies were further characterized using various biochemical tests (e.g., catalase test, oxidase reaction, growth on bile esculine [BE] agar, growth in the presence of 6.5% NaCl, growth at 45°C, motility, pyrrolidonyl arylamidase [PYR], yellow pigment, arginine dihydrolase [ADH], and sugars fermentation). Antibiotic susceptibility testing was done by standard disc diffusion method, according to the CLSI protocols. Detection of vancomycin-resistant Enterococcus (VRE) was performed by BHI agar screen plate.

Conclusions

Our data showed that resistance to vancomycin among Enterococcus spp. isolates is emerging. Knowledge of the causative microorganisms in DFIs and their antibiotic susceptibility profiles is essential for proper treatment and infection eradication.

Results

In the current study, a total of 86 diabetic patients were investigated. Enterococcus spp. were isolated from 34 (39.5%) patients consisting of 20 males (59%) and 14 females (41%). Twenty-five (73.5%) patients received antibiotic treatment on admission. Fifty (44.1%) cases had random blood sugar ranging between 130 - 300, and 19 (55.9%) had blood sugar of 300 - 450. Of the 34 patients, 15 (44.1%) had type 1 diabetes and 19 (55.9%) had type 2 diabetes. Enterococcus faecalis was the most common isolated Enterococcus spp. (50%). Linezolid was the most effective antibiotic against Enterococcus isolates, and ciprofloxacin was the least effective.

Background

Diabetic foot infections (DFIs) are an increasingly common public health problem and are associated with mortality and morbidity. The incidence of Enterococci in DFIs, a leading cause of hospital admission in Iran, has been increasing, possibly due to previous antibiotic use.

Objectives

The aims of this study were 1) isolation of bacteria from diabetic patients with foot ulceration, 2) characterization of the isolated bacteria, 3) confirmation of Enterococci and their genus, 4) determination of the susceptibility profile of the isolates, and 5) survey of the cross-resistance among Enterococcus spp.

Methods

A total of 86 diabetic patients with foot ulceration were investigated during 2012 - 2014 in Nemazee hospital (Shiraz, Iran). Swabs were collected from diabetic ulcers. For the isolation of bacteria, microbiological media were used. Colonies were further characterized using various biochemical tests (e.g., catalase test, oxidase reaction, growth on bile esculine [BE] agar, growth in the presence of 6.5% NaCl, growth at 45°C, motility, pyrrolidonyl arylamidase [PYR], yellow pigment, arginine dihydrolase [ADH], and sugars fermentation). Antibiotic susceptibility testing was done by standard disc diffusion method, according to the CLSI protocols. Detection of vancomycin-resistant Enterococcus (VRE) was performed by BHI agar screen plate.

Enterococcus spp.;Diabetic Foot Infections;Antibiotic Susceptibility;Antibiotic Cross-Resistance Enterococcus spp.;Diabetic Foot Infections;Antibiotic Susceptibility;Antibiotic Cross-Resistance http://www.pedinfect.portal.tools/index.php?page=article&article_id=37680 Mojtaba Anvarinejad Mojtaba Anvarinejad Prof. Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran Prof. Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran Gholamreza Pouladfar Gholamreza Pouladfar Prof. Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran Prof. Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran Aziz Japoni Aziz Japoni Prof. Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran Prof. Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran Shahram Bolandparvaz Shahram Bolandparvaz Head of Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Head of Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran Zeinab Satiary Zeinab Satiary General Surgery Ward, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran General Surgery Ward, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, IR Iran Jalal Mardaneh Jalal Mardaneh Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, IR Iran; Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, IR Iran Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, IR Iran; Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, IR Iran