Archives of Pediatric Infectious Diseases Archives of Pediatric Infectious Diseases Arch Pediatr Infect Dis http://www.pedinfect.portal.tools 2322-1828 2322-1836 10.5812/pedinfect en jalali 2018 8 16 gregorian 2018 8 16 5 2
en 10.5812/pedinfect.37968 Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of <italic>Escherichia coli</italic> Recovered from Patients with Urinary Tract Infections in Kerman, Iran research-article research-article Results

More than 50% of the isolates were multi-drug resistant. The prevalence of ESBLs, AmpC-β-lactamase, blaTEM and blaOXA in the inpatient isolates was 37.2%, 2%, 37.2% and 5.8%, respectively. Further, the prevalence of ESBLs, blaTEM, blaSHV and blaOXA in the outpatient isolates was 42.5%, 24%, 5.5% and 1.8%, respectively.

Conclusions

The prevalence of ESBL-producing E. coli strains in the community (outpatients) is higher than that in inpatients in Kerman, Iran. An outbreak of ESBL-producing isolates in the community can be a serious problem for public health, as resistance to other classes of antibiotics such as aminoglycoside and fluoroquinolones is often related with ESBL and AmpC production, therefore, detection of ESBL and AmpC-producing isolates in the community and hospitals is very important for the treatment and prevention of such isolates.

Background

Escherichia coli is the main causative pathogen in urinary tract infections (UTIs). Antibiotic resistance in this bacterium is an important problem in public health.

Objectives

The aim of this study was to identify the blaTEM, blaSHV, blaOXA, and blaPER genes and AmpC-β-lactamase in clinical isolates of E. coli recovered from patients with UTIs in Kerman, Iran.

Methods

E. coli isolates (N = 105) were analyzed for their antibiotic susceptibility with the disk diffusion method. ESBL and AmpC-producing isolates were detected using phenotypic methods. PCR was used to identify the blaTEM, blaSHV, blaOXA and blaPER genes in ESBL and AmpC-positive isolates.

Results

More than 50% of the isolates were multi-drug resistant. The prevalence of ESBLs, AmpC-β-lactamase, blaTEM and blaOXA in the inpatient isolates was 37.2%, 2%, 37.2% and 5.8%, respectively. Further, the prevalence of ESBLs, blaTEM, blaSHV and blaOXA in the outpatient isolates was 42.5%, 24%, 5.5% and 1.8%, respectively.

Conclusions

The prevalence of ESBL-producing E. coli strains in the community (outpatients) is higher than that in inpatients in Kerman, Iran. An outbreak of ESBL-producing isolates in the community can be a serious problem for public health, as resistance to other classes of antibiotics such as aminoglycoside and fluoroquinolones is often related with ESBL and AmpC production, therefore, detection of ESBL and AmpC-producing isolates in the community and hospitals is very important for the treatment and prevention of such isolates.

Background

Escherichia coli is the main causative pathogen in urinary tract infections (UTIs). Antibiotic resistance in this bacterium is an important problem in public health.

Objectives

The aim of this study was to identify the blaTEM, blaSHV, blaOXA, and blaPER genes and AmpC-β-lactamase in clinical isolates of E. coli recovered from patients with UTIs in Kerman, Iran.

Methods

E. coli isolates (N = 105) were analyzed for their antibiotic susceptibility with the disk diffusion method. ESBL and AmpC-producing isolates were detected using phenotypic methods. PCR was used to identify the blaTEM, blaSHV, blaOXA and blaPER genes in ESBL and AmpC-positive isolates.

Escherichia coli;MDR;ESBLs;AmpC Escherichia coli;MDR;ESBLs;AmpC http://www.pedinfect.portal.tools/index.php?page=article&article_id=37968 Maryam Koshesh Maryam Koshesh Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran Shahla Mansouri Shahla Mansouri Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran Zahra Hashemizadeh Zahra Hashemizadeh Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran Davood Kalantar-Neyestanaki Davood Kalantar-Neyestanaki Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran; Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran. Tel: +98-3433257665 Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran; Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, IR Iran. Tel: +98-3433257665